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Original Research Article | OPEN ACCESS

TRIM52 promotes proliferation, invasion, and migration of gastric cancer cells by regulating Wnt/β-catenin pathway

Beiyi Zheng1, Jianhua Wang1, Haozhen Wang1, Wenling Wang1, Yude Jin2

1Department of General Medicine, Jinhua People’s Hospital, Jinhua, Zhejiang Province 321000, China; 2Department of General Surgery, The First Affiliated Hospital of Huzhou University, Huzhou, Zhejiang Province 313000, China.

For correspondence:-  Yude Jin   Email: jinyude0120@163.com   Tel:+865722214540

Accepted: 28 April 2022        Published: 31 May 2022

Citation: Zheng B, Wang J, Wang H, Wang W, Jin Y. TRIM52 promotes proliferation, invasion, and migration of gastric cancer cells by regulating Wnt/β-catenin pathway. Trop J Pharm Res 2022; 21(5):957-963 doi: 10.4314/tjpr.v21i5.8

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: This study aimed to reveal the role and mechanism of tripartite motif-containing 52 (TRIM52) in gastric cancer (GC) progression.
Methods: The Cancer Genome Atlas (TCGA) database was utilized to analyze TRIM52 expression in GC samples and para-carcinoma tissue samples, and the results were confirmed by quantitative real-time polymerase chain reaction. Cell counting kit-8 and colony formation assays were used to evaluate cell viability. Wound healing assay was utilized to analyze cell migration, while Transwell assay was utilized to evaluate cell invasion. TRIM52, proliferating cell nuclear antigen, matrix metalloproteinase-2, Wnt5a, β-catenin, and c-Myc protein levels were measured by western blot.
Results: TRIM52 was expressed more in GC tissue samples and cells compared to normal tissues and cells (p < 0.001). Overexpression of TRIM52 promoted growth, migration, and invasion of HGC-27 cells, and silencing inhibited growth, migration, and invasion of HGC-27 cells (p < 0.001). In addition, TRIM52 overexpression increased Wnt5a, β-catenin, and c-Myc protein expression, and silencing decreased Wnt5a, β-catenin, and c-Myc protein expression (p < 0.001 or p < 0.01), indicating that TRIM52 activates Wnt/β-catenin signaling pathway.
Conclusion: These findings reveal that TRIM52 facilitates GC cell proliferation, migration and invasion, but activates Wnt/β-catenin signaling.

Keywords: TRIM52, Gastric cancer, Migration, Invasion, Wnt/β-catenin signaling pathway

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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